Dr.Piyanuch Sornchai

คณาจารย์ผู้ทรงคุณวุฒิ

 ดร.ปิยนุช ศรชัย

คณาจารย์และผู้ทรงคุณวุฒิ

โครงการ Mini Entrepreneur

EDUCATION

2016 Post-Doctoral at UTAH State University

2015 Doctor of Philosophy (Agricultural Biotechnology 

Kasetsart University, Kamphaeng Saen, Thailand: GPA 3.87 Thesis title: “The biosafety assessment of genetically modified Dendrobium orchid possess antisense CP-ACO1 gene under screen house condition”

2009 Master of Science (Agricultural Biotechnology)

Kasetsart University, Kamphaeng Saen, Thailand: GPA: 3.57 Thesis title: “The expression of antisense papaya 1-aminocyclopropane-1-carboxylic acid oxidase and the physiological study of transgenic Dendrobium orchids”

2005 Bachelor of Science (Agricultural production) 

Thammasat University, Thailand: GPA: 3.18 Senior project title: “Effects of bioextracts on growth and yield of papaya”

SCHOLARSHIP/AWARD

December 2012 The award of outstanding in Oral presentation at The 5thAG-BIO/PERDO Graduate Conference, Thailand.

Topic : “Agrobacterium removing test and the presence of antisense CPACO gene in transgenic”

March 2012 Short-Term Scholarship from University of TSUKUBA, Japan Skill practice : Tissue culture technique

December 2010 The award of outstanding in Oral presentation at The 4thAG-BIO/PERDO Graduate Conference, Thailand.

Topic :“Ethylene production and vase life of transgenic Dendrobium orchid inflorescence possessed CPACO antisense gene”

May 2009 The award of outstanding in Oral presentation at The 8thNational Horticultural Congress 2009, Thailand.

Topic : “The ethylene production of transgenic Dendrobiumorchid possessed CPACOantisene gene”

 March 2004 Short-Term Scholarship from University of TSUKUBA, Japan

Skill practice : Tissue culture technique

October 2003 Short-Term Scholarship from KOIBUCHI College, Japan

Skill practice : Farm practice and Tissue culture technique

March - May 2002 Short-Term Scholarship from TOKYO NODAI University, Japan

Skill practice : Farm and Machine practice

PROFESSIONAL EXPERIENCES
 

January 2017- present Researcher: Biotechnology and Development Office, Ministry of  Agriculture, Department of Agriculture, Thailand

October-December 2016 Researcher (Agricultural Biotechnology and Plant Breeding) 

Plants, Soils and Climate: Utah State University, USA.

January - August 2016 Post-Doctoral  Researcher (Agricultural Biotechnology) 

Center for Advanced Studies for Agriculture and Food, (CASAF, NRU-KU), Kasetsart University Institute for Advanced Studies, Kasetsart University, Bangkok, Thailand

Project title: “The evaluation of the existence of antisense CPACO gene in micro-propagule of transgenic  Dendrobium”

May 2010 - June 2015  Teaching Assistance (TA):

Interdisciplinary Program in  Agricultural  Biotechnology,  Faculty of Agriculture at KamphaengSaen, Kasetsart  University, Kamphaeng Sean Campus Skill practice : basic molecular techniques i.e. DNA extraction, PCR preparation and analysis

October 2007 and 2012 Teaching Assistance(TA):

Molecular Biology Laboratory Class, The Center for Agricultural Biotechnology, KasetsartUniversity, Kamphaeng Sean Campus Skill practice : molecular biology and transformation techniques

September-October 2009 The English Supplementary Program in  Advance English for Conservation, Discussion and Career Purposes. Graduate School, KU Skill practice : English literacy

April 2006 - 2015 Research Assistance(RA):

Plant Cell, Tissue  and Transformation  Laboratory, The  Center  for  Agricultural  Biotechnology,Kasetsart University, Kamphaeng Sean Campus Skill practice : Plant tissue culture, plant transformation via particle bombardment and Agrobacterium-mediated transformation techniques

6 May 2004 - 2005 Trainee:

Tissue Culture and Hydroponic Laboratory, Faculty of Science and Technology, Thamasat University Skill practice : Plant tissue culture technique and Hydroponic Culture

PUBLICATIONS/PRESENTATIONS

Publications

  1.  Sornchai, P., R. Koto and S. Chanprame. 2011. Techniques and parameters effecting the transformation efficiency of particle bombardment and Agrobacterium-mediated transformation in Dendrobium orchid. Agricultural Sci. J.42(2): 255-264.

  2. Sornchai, P., R. Koto, W. Imsabai, P. Burn, S. Chanprame, and S. Chanprame. 2015. Genetic Transformation of Dendrobium 'Sonia Earsakul' with Antisense Carica papaya ACO1 Gene. Modern Applied Sci. 9 (12): 125-133.

  3. Sornchai, P. W. Khampong and S. Chanprame. 2016. The nuclear DNA content and pollen viability of 25 Dendrobium cultivars. Agricultural Sci. J. 47(2): 227-240.

  4. Rodjanawijid, S., P. Sornchai, D.Sumrittinun, N. Dechsangkrano, B. Kongsamai and S.Chanprame. 2016. The suitable pollinia separation and germination technique for certain Dendrobium orchid cultivars. Agricultural Sci. J. 47(3): 305-316.

  5. Sornchai, P., P. Burn and S. Chanprame.2007. Ethylene Production in the vegetative part of Transgenic Dendrobium Orchid Possessed cpACO Antisense Gene.in 3rdAgBiotech Graduate Conference. Kasetsart University Kamphaeng Saen Campus, Nakhon Pathom, Thailand.

  6. Sornchai, P., W. Imsabai, P. Burn and S. Chanprame. 2009. The Ethylene Production of Transgenic Dendrobium Orchid Possessed cpACO Antisense Gene. The 8th National Horticultural Congress. Maejo University, Chiang mai, Thailand.

  7. Sornchai, P., W. Imsabai, P. Burn and S. Chanprame. 2011. Ethylene Production and Vase life of Transgenic Dendrobium 'Earsakul' Inflorescence Possessed Antisense CP-ACO Gene. in 4thAgBiotech Graduate Conference. Kasetsart University Kamphaeng Saen Campus, Nakhon Pathom, Thailand.

  8. Sornchai, P., S. Chanprame and K. Watanabe. 2012. Agrobacterium removing test and the presence of antisense CPACO gene in transgenic Dendrobium orchid. in 5th AgBiotech Graduate Conference. Kasetsart University Kamphaeng Saen Campus, Nakhon Pathom, Thailand.

  9. Sornchai, P., P. Burn, S. Chanprame and S. Chanprame. 2014. Assessing the effect of transgenic Dendrobium orchid on rhizosphere bacterial communities. in 6th AgBiotech Graduate Conference. Kasetsart University Kamphaeng Saen Campus, Nakhon Pathom, Thailand.50pp.

  10. Rodjanawijid S., Sornchai, P and S. Chanprame. 2014. The molecular investigation and the morphological study of transgenic Dendrobium orchid possessed gus reporter gene. in 6th AgBiotech Graduate Conference. Kasetsart University Kamphaeng Saen Campus, Nakhon Pathom, Thailand. 49pp.

  11. Sornchai, P.and S. Chanprame. 2011. Transgenic Dendrobium 'Sonia' Earsakul Possessed Antisense CPACO Gene Exhibit Similar Morphological Characters to the Non-transgenic Line. P 63 in ISSASS International Symposium and Congress 2011. Bogor, Indonesia.

  12. Chanprame, S. and P. Sornchai. 2012. The confirmation of no horizontal gene transfer from transgenic Dendrobium orchids to rhizosphere bacteria. P 262 in ISSASS International Symposium and Congress 2012. Albay, Philippines.

  13. Sornchai, P.and S. Chanprame. 2012. The antisense CPACO gene from papaya in transgenic Dendrobium orchid caused lower ACC oxidase activity. P 256 in ISSASS International Symposium and Congress 2012. Albay, Philippines.

  14. Chanprame, S. and P. Sornchai. 2013. The rhizosphere bacterial communities surrounding transgenic and non-transgenic Dendrobium orchids are proved similar. P 22 in ISSASS International Symposium and Congress 2013. Acacia Hotel Manila, Philippines.

  15. Sornchai, P.and S. Chanprame. 2013. Pollen quality of transgenic Dendrobium orchids is similar to non-transgenic line. P 23 in ISSASS International Symposium and Congress 2013. Acacia Hotel Manila, Philippines.

  16. Khampong, W., P. Sornchai and S. Chanprame. 2014. Nuclear DNA content of 25 Thai commercial Dendrobium cultivars analyzed by flow cytometry. P45 in 14th International Student Summit 2014. Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, Thailand.

  17. Ruangsomboona, S., P. Sornchai and N. Prachoma.2018. Enhanced hydrocarbon production and improved biodiesel qualities of Botryococcus braunii KMITL 5 by vitamins thiamine, biotin and cobalamin supplementation. Agal Research. 29(2018:159-169.

  18. Sornchai, P., T. chookaew, N. Kaewnuy, T. Assawamongkolsiri and K. Wongwathanarat. 2018 Developing of Genetically Modified Maize, Mon810 and NK603 Detection Method using Multiplex Real-time PCR technique. The 15th KU-KPS National Conference Proceeding.2945pp:27-40.

  19. Thammakijjawat, P., P. Sornchai,T. Assawamongkolsiri and P. Sanvittayakul. 2018. Development triplex real-time PCR screening method to detect GM maize for ISO/IEC17025 standard accreditation. Thai agricultural research journal. Vol36(3):316-331

  20. Sornchai, P., T. chookaew, N. Buntongdee, T. Assawamongkolsiri and K. Wongwathanarat. 2019 Validation of the Method for Event Specific Mon810 and NK603 and Endogenous Gene Identification by using Multiplex Real-time PCR Technique. Thai Agricultural Research Journal. 37:224-237

  21. Thitirut Assawamongkholsiri, Piyarat Thammakijjawat, Thanawadee Leejarkpai ,Piyanuch Sornchai, Parinda Songmuangsook, Weerasak Pitaksaringkarn and Nattawadee Buntongdee. Development of Kanamycin Adhesive Tape for Screening nptII in Transgenic Papaya. In The 31st Annual Meeting of the Thai Society for Biotechnology and International Conference November 10-12, 2019

  22. Sornchai, P., N. Buntongdee, T. Assawamongkolsiri, P. Songmuangsook, W. Pitaksaringkarn and P. Thammakijjawat. 2020. Development of Screening Method for CaMV 35S promoter, Nos terminator, NptII and Papaya Endogenous Gene in GM Papaya  using Tetraplex Real-time PCR. The 17th KU-KPS National Conference Proceeding. 5553pp:85-96.

  23. Sornchai. P., W.G. vanDoorn, W. Imsabai, P. Burns and S. Chanprame. 2020. Dendrobium orchids carrying antisense ACC oxidase: Small changes in flower morphology and a delay of bud abortion, flower senescence, and abscission of flowers. Transgenic Research. 29.429-442.

Presentations

  1. Sornchai, P., P. Burn and S. Chanprame. 2007. Ethylene Production in the vegetative part of Transgenic Dendrobium Orchid Possessed cpACO Antisense Gene.in 3rd AgBiotech Graduate Conference. Kasetsart University Kamphaeng Saen Campus, Nakhon Pathom, Thailand.
  2. Sornchai, P., W. Imsabai, P. Burn and S. Chanprame. 2009. The Ethylene Production of Transgenic Dendrobium Orchid Possessed cpACO Antisense Gene. The 8th National Horticultural Congress. Maejo University, Chiang mai, Thailand.
  3. Sornchai, P., W. Imsabai, P. Burn and S. Chanprame. 2011. Ethylene Production and Vase life of Transgenic Dendrobium 'Earsakul' Inflorescence Possessed Antisense CP-ACO Gene. in 4th AgBiotech Graduate Conference. Kasetsart University Kamphaeng Saen Campus, Nakhon Pathom, Thailand.
  4. Sornchai, P., S. Chanprame and K. Watanabe. 2012. Agrobacterium removing test and the presence of antisense CPACO gene in transgenic Dendrobium orchid. in 5th AgBiotech Graduate Conference. Kasetsart University Kamphaeng Saen Campus, Nakhon Pathom, Thailand.
  5. Sornchai, P., P. Burn, S. Chanprame and S. Chanprame. 2014. Assessing the effect of transgenic Dendrobium orchid on rhizosphere bacterial communities. in 6th AgBiotech Graduate Conference. Kasetsart University Kamphaeng Saen Campus, Nakhon Pathom, Thailand.50pp.
  6. Rodjanawijid S., Sornchai, P and S. Chanprame. 2014. The molecular investigation and the morphological study of transgenic Dendrobium orchid possessed gus reporter gene. in 6th AgBiotech Graduate Conference. Kasetsart University Kamphaeng Saen Campus, Nakhon Pathom, Thailand. 49pp.
  7. Sornchai,  P. and S. Chanprame. 2011. Transgenic Dendrobium 'Sonia' Earsakul Possessed  Antisense CPACO Gene Exhibit Similar Morphological Characters to the Non-transgenic Line. P 63 in ISSASS International Symposium and Congress 2011. Bogor, Indonesia.
  8. Chanprame, S. and P. Sornchai. 2012. The confirmation of no horizontal gene transfer from transgenic Dendrobium orchids to rhizosphere bacteria. P 262 in ISSASS International Symposium and Congress 2012. Albay, Philippines.
  9. Sornchai,  P. and S. Chanprame. 2012. The antisense CPACO gene from papaya in transgenic Dendrobium orchid caused lower ACC oxidase activity. P 256 in ISSASS International Symposium and Congress 2012. Albay, Philippines.
  10. Chanprame, S. and P. Sornchai. 2013. The rhizosphere bacterial communities surrounding transgenic and non-transgenic Dendrobium orchids are proved similar. P 22 in ISSASS International Symposium and Congress 2013. Acacia Hotel Manila, Philippines.
  11. Sornchai,  P. and S. Chanprame. 2013. Pollen quality of transgenic  Dendrobium orchids is  similar to non-transgenic line. P 23 in ISSASS International Symposium and Congress 2013. Acacia Hotel Manila, Philippines.
  12. Khampong, W., P. Sornchai and S. Chanprame. 2014. Nuclear DNA content of 25 Thai commercial  Dendrobium cultivars analyzed by flow cytometry. P45 in 14th International Student Summit 2014. Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, Thailand.
  13. Chanprame, S., P. Sornchai, S. Reanboon, W. Kongkuna and W. Imsabai. 2018. The transgenics Dendrobium 'Sonia' Bom17 containing antisense ACO gene demonstrated normal growth with prolong vase life. in The 3rd International Orchid Symposium, Seoul, Korea.

ACTIVITIES
  • Staff of Institution Biosafety Committee of Department of Agriculture
  • Representative of Department of Agriculture Thailand: The 17th Meeting of the ASEAN GM Food Testing Network, Thailand, June-July 2020.
  • Representative of Department of Agriculture Thailand: The 16th Meeting of the ASEAN GM Food Testing Network, Malaysia, April 2019.
  • Representative of Department of Agriculture Thailand: The 14th Meeting of the ASEAN GM Food Testing Network, Myanmar, July 2017.
  • Head of organizing committee: 6thAG-BIO/PERDO Graduate Conference on Agricultural Biotechnology and KU-UT joint Seminar II, Thailand, December 2014
  • Staff: the Biosafety on GM plants workshop of  Biotechnology and Biosafety Information Center, Thailand,
  • Organizing committee: 5thAG-BIO/PERDO Graduate Conference on Agricultural Biotechnology and KU-UT joint Seminar II, Thailand, December 2012
  • Staff: the 6th International Workshop on Management of the Diamondback Moth and Other Crucifer Insect Pests, Thailand, March 2011
  • Organizing committee: 4thAG-BIO/PERDO Graduate Conference on Agricultural Biotechnology and KU-UT joint Seminar, Thailand, December 2010

TECHINICAL SKILLS

Advanced knowledge of the following skills:

  • Scientific skill: Plant Tissue Culture, Plant transformation, Plant Molecular Biology, Post-harvest technology
  • Computer skill: Microsoft Office [Word, Excel, Power point], Internet, Phylogenetic program
  • English skill: good speaking, good reading, good writing
  • Japanese skill :speaking reading and writing: good with the certificate of JAPANESE –LANGUAGE PROGICIENCY Level 3(2009)